Flow Cytometry Services

Our facility maintains a CLIA-certified laboratory that provides processing and flow cytometry analysis services for diagnostic patient samples and clinical experimental protocols. The lab receives samples from all over the nation. For available tests, click here.

Flow Cytometry Resource Facility

Our Research Flow Cytometry facility houses 5 flow analyzers that are accessible 24 hours a day, 7 days a week to all investigators, as well as 2 cell sorters and several breadboard instruments that are operator-assisted and available during regular weekday business hours (8:00 A.M. to 6:00 P.M. Monday – Friday).

Flow Analyzers

In the 24/7 accessible user room we currently have:

  • FACSCalibur 1 (488 laser and 635 nm diode, 4 fluorescent detectors);
  • FACSCalibur 2 (488 laser and 635 nm diode, 4 fluorescent detectors);
  • LSRII-A: equipped with five lasers (355 nm, 408 nm, 488 nm, 561 nm, and 640 nm) and 20 fluorescent PMT's;
  • LSRII-B: equipped with four lasers (408 nm, 488 nm, 561 nm, and 640 nm) and 18 fluorescent PMT's;
  • LSRFortessa (408 nm, 488 nm, and 640 nm lasers) with 14 fluorescent PMT's.

During weekdays, staff will perform start-up procedures and daily QC on these instruments. Users can sign up for time on the equipment using a web-based calendar system. For new user accounts, please contact us with the required information. Principal investigators are billed for booked calendar time and usage time at the end of each month. For more information on billing policies, click here.

In our experience, there are three types of research investigators who use the Flow Cytometry Resource:

  • Users already proficient in flow cytometry who know its capabilities and can use the equipment and associated data analysis software independently. Facility staff is available during regular business hours to provide oversight and consultation as required.
  • Users new to flow cytometry but interested in becoming proficient. Staff will help pilot their studies and then train the personnel to setup, run and analyze their experiments. Staff then works one-on-one to train the investigators on proper sample preparation, instrument operation and data analysis for their protocols. Our goal is to train these investigators to become proficient in the technique and work in the facility independently.
    Users new to flow cytometry or specific instrumentation are requested to contact us to set up an appointment during regular business hours, so that we can properly assist you with setting up your experiments. Several courses are available for free to aid in gaining proficiency on the instruments.
  • Users depending exclusively on the Resource to provide sample preparation, data acquisition, analysis and interpretation. This service is fee-based and generally available by arrangement from 8:00 A.M. to 6:00 P.M. Monday through Friday.

Cell Sorters

The Flow Sorting Facility houses two cell sorter instruments, FACSAria I and FACSAria II. The FACSAria I was upgraded in September 2010 and is equipped with 18 fluorescent PMT's and 405, 488, 561, and 640 nm lasers, and has bio-containment capabilities. The FACSAria II is equipped with four lasers (355 nm, 408 nm, 488 nm, and 633 nm) and has 13 fluorescent detectors. Both instruments have the capacity to do four-way sorts as well as sorting into microtiter plates (96-, 48-, 24-, 12-, or 6-wells) and onto slides. The instruments are operated by facility staff members and available during regular business hours by arrangement only. For more information consult the Sorting facility website.

Breadboard Flow Cytometers

The Flow and Imaging Electronics Shop provides a unique service to the core facility. The modestly equipped shop has the ability to service, repair modify or construct flow cytometers utilized by Roswell staff. Custom technology available for studies includes contiguous multispectral detection using diffraction grating optics, Infra Red flow cytometry, fluorescence detection beyond 1100 nm and an optical Breadboard flow cytometer open to diverse configurations. Researchers interested in using this technology are to contact Ed Posniesinski (x8775).